Laboratory Introduction
实验室
实验室
基金。
Biorefinery
of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative
of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen
with many desirable characteristics, which makes it an ideal industrial microbial
biocatalyst for commercial production of desirable bioproducts through metabolic
engineering.
In this study,
we determined the complete chromosome and plasmid sequences of ZM4 and its engineered
xylose-utilizing derivatives 2032 and 8b. Compared to previously published and revised
ZM4 chromosome sequences, the ZM4 chromosome sequence reported here contains 65
nucleotide sequence variations as well as a 2400-bp insertion.
Pretreatment
is the key step to overcome the recalcitrance of lignocellulosic biomass making
sugars available for subsequent enzymatic hydrolysis and microbial fermentation.
During the process of pretreatment and enzymatic hydrolysis as well as fermentation,
various toxic compounds may be generated with strong inhibition on cell growth and
the metabolic capacity of fermenting strains.
The aim of this
work was to identify inhibitors in pretreated lignocellulosic slurries, evaluate
high-throughput screening strategies, and investigate the impact of inhibitors on
potential hydrocarbon-producing microorganisms. Compounds present in slurries that
could inhibit microbial growth were identified through a detailed analysis of saccharified
slurries by applying a combination of approaches of high-performance liquid chromatography,
GC-MS, LC-DAD-MS, and ICP-MS.